Cat. No | MBA-049 |
Name | Rabbit anti-Substance P Antibody |
中文名字 | 兔抗P物质抗体 |
Description | The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000-1/8000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Bird, Bullfrog, Cat, Chick, Chicken, Dog, Dolphin, Doves, Equine, Fish, Frog, Gerbil, Goat, Goldfish, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Mudpuppy, Narwhal, Parrot, Pig, Pig, Pigeon, Quail, Rabbit, Ram (Sheep), Rana Pipiens (Leopard Frog), Rat, Ratfish, Salmon, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Snail, Sparus Aurata (Fish), Squalus Acanthias (Dogfish), Squirrel, Starfish, Sting Ray, Sturgeon (Fish), Three-Spined Stickleback (Fish), Trout, Turtle, Vole, Zebra Finch, Zebrafish |
Preabsorption Control | / |
Alternate Names | Protachykinin-1; PPT; tachykinin peptide hormone; NKA, NKNA, TAC2; tachykinin, precursor 1, anti-substance P |
RRID | AB_572266 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | TAC1 |
Entrez Gene ID | Entrez Gene: 100227778 Bird |
NCBI Gene Aliases | / |
Sequence | 33507-63-0 |
APPLICATION | |
Quality Control | The Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant positive labeling of rat substantia nigra and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Specificity was demonstrated using soluble preadsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Immunolabeling was completely abolished by preadsorption with Substance P. Preadsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K. |
Tissue | Rat spinal cord (dorsal horn) and substantia nigra |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL for 20-30 min. |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by the manufacturers. |
Suggested Dilution | 1/6,000 – 1/8,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles |
