Cat. No.
MABL-3385
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AFD-19C5
From
Recombinant Antibody
Specificity
This antibody binds an enzyme called indoleamine 2,3-dioxygenase 1 (IDO1). This molecule catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. It is involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. It acts as a suppressor of anti-tumor immunity. IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral and bacterial infections.
Alternative Names
IDO-1; IDO; INDO; Indoleamine 2 3-dioxygenase 1; Indoleamine-pyrrole 2 3-dioxygenase; CGMCC NO 20770; EPR20374
UniProt
P14902
Immunogen
The original antibody was generated by immunizing mice with recombinant human IDO1 protein.
Application Notes
This antibody can bind human IDO1 in an ELISA. The original mouse IgG1 antibody binds human IDO1 with an affinity constant of 7.68 X 10^(9) M^(-1). This antibody can be used for the immunohistochemical analysis of various normal and cancerous human tissues like lymph node cells and Hodgkin's lymphoma tissues. The immunohistochemical staining pattern of this antibody is similar to another anti-IDO1 antibody EPR20374 (CN113045667).
Antibody First Published
Note on publication
Describes the generation of two antibodies against major cat allergen Fel d 1 and their possible use in treating cat allergies using allergen-specific immunotherapy (SIT).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
