Cat. No.
MAK-0148
Product Name
Cat CRP
Storage
2-8°C
Species
Cat
Introduction
CRP is an acute phase protein that is elevated in the serum of many mammalian species because of injury, infection, or disease. CRP levels can increase several hundred-fold in humans, monkeys and dogs. In cats, CRP is considered a more modest acute phase reactant.
Principle Of The Assay
The assay uses affinity purified cat CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated cat CRP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
