Cat. No.
MAK-0149
Product Name
Pig CRP
Storage
4°C
Species
Pig
Introduction
CRP is an acute phase protein that is elevated in serum because of injury, infection, or disease. Baseline levels of CRP in pigs range from 5-30 µg/ml. Levels may increase to 500 µg/ml during the acute phaseresponse. Measurement of CRP provides a convenient marker of inflammation and disease.
Principle Of The Assay
The assay uses affinity purified pig CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated pig CRP antibodies for detection. Standards and diluted samples are incubated in wells of a 96-well plate for 45 minutes. The wells are subsequently washed. HRP conjugate, prepared from a concentrated stock is added, and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
