Cat. No.
MAK-0163
Product Name
Rat Fibrinogen
Storage
4°C
Species
Rat
Introduction
Fibrinogen is dimeric protein (mwt, 340 kDa), that is synthesized in the liver and circulates in rat plasma at a concentration of approximately mg/ml. It is significantly elevated during the acute phase response and therefore serves as a useful marker of infection, disease, and inflammation.
Principle Of The Assay
The assay uses affinity purified rat fibrinogen antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat fibrinogen antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 30 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 30 minutes. This results in fibrinogen molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If fibrinogen is present a blue color develops. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of fibrinogen is proportional to absorbance and is derived from a standard curve.
