Cat. No.
MAK-0164
Product Name
Dog Gastrotropin
Storage
4°C
Species
Dog
Introduction
Gastrotropin (GTP), also known as ileal fatty acid binding protein, and FABP6, is a cytoplasmic protein involved in intestinal fatty acid transport.
Principle Of The Assay
One is used as coating antibody. The other is conjugated to HRP and used for detection. Standards and diluted samples (100 μl) are incubated in the coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 μl) is added and incubated for 45 minutes. If gastrotropin molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If gastrotropin is present, a blue color develops. Color development is stopped after 20-minutes by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of gastrotropin is proportional to absorbance and is derived from a standard curve.
