Cat. No.
MAK-0174
Product Name
Alpaca Haptoglobin
Storage
2-8°C
Species
Alpaca
Introduction
Haptoglobin is an acute phase protein that is elevated in the blood of most mammals during infection and inflammation have reported that serum haptoglobin levels increase in alpacas after exposure to lipopolysaccharide, which induces an acute phase response.
Principle Of The Assay
The assay uses affinity purified alpaca haptoglobin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated alpaca haptoglobin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed.
HRP conjugate is added and incubated for 45 minutes. This results in haptoglobin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If haptoglobin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of haptoglobin is proportional to absorbance and is derived from a standard curve.
