Cat. No.
MAK-0175
Product Name
Trout/Salmon Haptoglobin
Storage
4°C
Species
Trout/Salmon
Introduction
Haptoglobin-like protein was identified in proteomic studies as a protein that is increased in Atlantic salmon with cardiomyopathy syndrome and inflammation. It was also found to be upregulated in rainbow trout after challenge with Flavobacterium psychrophilum.
Principle Of The Assay
The assay uses a polyclonal antibody that recognizes Atlantic salmon and rainbow trout haptoglobin. Unconjugated antibody is coated on wells of a microtiter plate and used for capture. Horseradish peroxidase (HRP) conjugated antibody is used for detection. Standards and diluted samples (100 μl) are incubated in the antibody coated microtiter wells for one hour. After washing the wells, HRP-conjugate (100 μl) is added and incubated for 45 minutes. If haptoglobin molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If haptoglobin is present, a blue color develops. Color development
is stopped by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of haptoglobin is proportional to absorbance and is derived from a standard curve.
