Cat. No.
MAK-0176
Product Name
Mouse Hemopexin
Storage
4°C
Species
Mouse
Introduction
Hemopexin is an acute phase protein that is elevated up to 4-fold in mouse serum and plasma because of inflammation and infection. Hemopexin provides a convenient marker of inflammation and tissue injury in mice.
Principle Of The Assay
The assay uses affinity purified mouse hemopexin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse hemopexin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in hemopexin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound
HRP-conjugate and TMB is added and incubated for 20 minutes. If hemopexin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow.
Absorbance is measured at 450 nm. The concentration of hemopexin is proportional to absorbance and is derived from a standard curve.
