Cat. No.
MAK-0178
Product Name
Chicken Hemopexin
Storage
4°C
Species
Chicken
Introduction
Hemopexin (Hpx) is an acute phase protein of approximately 60 kDa. It is synthesized in the liver and circulates in blood. We also found that samples with elevated Hpx also had increased levels of the acute phase proteins alpha-1-acid glycoprotein and serum amyloid A.
Principle Of The Assay
The assay uses two chicken Hpx antibodies; one for solid-phase immobilization (microtiter wells) and one, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in Hpx molecules being sandwiched between the immobilization and detection antibodies. The wells are washed to remove unbound HRP-conjugate and TMB is added and
incubated for 20 minutes. If Hpx is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of Hpx is proportional to absorbance and is derived from a standard curve.
