Cat. No.
MAK-0182
Product Name
Chicken Ex-FABP
Storage
4°C
Species
Chicken
Introduction
Chicken extracellular fatty acid binding protein (Ex-FABP) is an antibacterial siderophore binding lipocalin that is expressed in serum and egg white. Proteomic studies indicate that it behaves as a positive acute phase protein; serum levels increase after lipopolysaccharide challenge.
Principle Of The Assay
Standards and diluted samples are incubated in the microtiter wells with HRP-conjugate for one hour. This results in Ex-FABP molecules being sandwiched between the immobilization and detection antibodies. The wells are washed and TMB is added and incubated for 20 minutes. If Ex-FABP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of Ex-FABP is proportional to absorbance and is
derived from a standard curve.
