Cat. No.
MAK-0181
Product Name
Human LRG1
Storage
2-8°C
Species
Human
Introduction
Leucine-rich alpha-2-glycoprotein-1 (LRG1) is a 50 kDa serum protein that is primarily expressed in liver. It is a positive acute phase reactant; serum levels are elevated during chronic inflammatory diseases and infections. It is also reported that LRG1 is upregulated in patients with ovarian and hepatocellular cancer.
Principle Of The Assay
The assay uses affinity purified human LRG1 antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated human LRG1 antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in LRG1 molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRPconjugate and TMB is added and incubated for 20 minutes. If LRG1 is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of LRG1 is proportional to absorbance and is derived from a standard curve.
