Cat. No.
MAK-0180
Product Name
Cow Lactoferrin
Storage
4°C
Species
Cow
Introduction
Lactoferrin is a non-heme iron binding glycoprotein found in milk, other secretory fluids, and blood. As a component of host defense, it has antimicrobial and anti-inflammatory activity. It is a biomarker of mastitis in cattle.
Principle Of The Assay
The assay uses affinity purified cow lactoferrin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated cow lactoferrin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in lactoferrin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound
HRP-conjugate and TMB is added and incubated for 20 minutes. If lactoferrin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow,
and absorbance is measured at 450 nm. The concentration of lactoferrin is proportional to absorbance and is derived from a standard curve.
