Cat. No.
MAK-0184
Product Name
Rat Murinoglobulin
Storage
2-8°C
Species
Rat
Introduction
Murinoglobulin is a negative acute phase reactant, the levels of which decrease in rat serum or plasma because of injury, infection or disease. In a rat adjuvant induced arthritis model, murinoglobulin levels decrease by approximately eight fold.
Principle Of The Assay
The assay uses affinity purified rat murinoglobulin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat murinoglobulin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 30 minutes. This results in murinoglobulin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound
HRP-conjugate and TMB is added and incubated for 20 minutes. If murinoglobulin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of murinoglobulin is proportional to absorbance and is derived from a standard curve.
