Cat. No.
MAK-0185
Product Name
Mouse α-Macroglobulin
Storage
2-8°C
Species
Mouse
Introduction
α-Macroglobulin is a serum proteinase inhibitor that consists of two major and one minor polypeptide chains. It is a negative acute phase reactant, the levels of which decrease in mouse serum or plasma during inflammation.
Principle Of The Assay
The assay uses affinity purified mouse α-macroglobulin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse α-macroglobulin antibodies for
detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in α-macroglobulin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If α-macroglobulin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of α-macroglobulin is proportional to absorbance and is derived from a standard curve.
