Cat. No.
MAK-0187
Product Name
Pig MAP
Storage
4°C
Species
Pig
Introduction
Pig Major Acute-Phase Protein (MAP), serum levels increase significantly during inflammatory conditions .
Principle Of The Assay
One is used as coating antibody. The other is conjugated to HRP and used for detection. Standards and diluted samples (100 μl) are incubated in anti-MAP coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 μl) is added and incubated for 45 minutes. If MAP molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If MAP is present, a blue color develops. Color development is stopped after 20-minutes by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of MAP is proportional to absorbance and is derived from a standard curve.
