Cat. No.
MAK-0188
Product Name
Monkey MPO
Storage
4°C
Species
Monkey
Introduction
Myeloperoxidase (MPO) is predominantly expressed in neutrophils. At sites of infection or inflammation, neutrophils degranulate and release MPO. By virtue of its peroxidase activity, it generates anti-microbial hypohalous acids. In humans it serves as a biomarker of infectious diseases and inflammatory conditions.
Principle Of The Assay
MPO-20-4D1 is used as coating antibody and HRP conjugated MPO-20-7A8 is used for detection. Standards and diluted samples (100 µl) are incubated in anti-MPO coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 µl) is added and incubated for 45 minutes. If MPO molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If MPO is present, a blue color develops. Color development is stopped after 20-minutes by addition of Stop Solution, changing the color to yellow.
Absorbance is measured at 450 nm. The concentration of MPO is proportional to absorbance and is derived from a standard curve.
