Cat. No.
MAK-0189
Product Name
Human Neutrophil Elastase
Storage
2-8°C
Species
Human
Introduction
Neutrophil elastase (NE) is a serine protease that is secreted by neutrophils. Increased serum levels have been associated with colorectal cancer. Increased sputum levels have been found in COPD patients with bacterial infection and in patients with bronchiectasis.
Principle Of The Assay
The NE ELISA uses affinity purified NE antibodies for solid phase immobilization (microtiter wells) and horseradish peroxidase (HRP) conjugated NE antibody for detection. Standards and diluted samples are incubated in the microtiter wells for one hour. The wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. NE molecules are thereby sandwiched between the immobilization and detection antibodies. The wells are washed to remove unbound HRP-conjugate, and TMB reagent is
added and incubated for 20 minutes. A blue color develops if NE is present. The assay is stopped by the addition of Stop Solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of NE is derived from a standard curve.
