Cat. No.
MAK-0190
Product Name
Dog PAP-1
Storage
4°C
Species
Dog
Introduction
Pancreatitis-associated protein-1 (PAP-1) is a 16.5 kDa protein that is expressed at high levels during inflammatory bowel disease (IBD) in humans.
Principle Of The Assay
The assay uses two dog PAP-1 antibodies, one for solid phase immobilization and one conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples (100 µl) are incubated in antibody coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 µl) is added and incubated for 45 minutes. PAP-1 molecules, if present, are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If PAP-1 is present, a blue color develops. Color development is stopped by addition of stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The
concentration of PAP-1 is proportional to absorbance and is derived from a standard curve.
