Cat. No.
MAK-0191
Product Name
Cat Paraoxonase
Storage
4°C
Species
Cat
Introduction
Paraoxonase 1 (PON1) is an enzyme that is synthesized in liver and secreted into blood, where it associates with HDL. It hydrolyses organophosphates but its natural substrates are currently unknown. It has been reported that PON1 levels, as measured by enzyme activity, decrease in cats with feline infectious peritonitis .
Principle Of The Assay
The assay uses polyclonal antibodies generated against recombinant cat PON1. Standards and dissociated/diluted samples (100 μl) are incubated in anti-PON1 coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 μl) is added and incubated for 45 minutes. If PON1 molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If PON1 is present, a blue color develops. Color development is stopped after 20minutes by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of PON1 is proportional to absorbance and is derived from a standard curve.
