Cat. No.
MAK-0202
Product Name
Cow SAA3 (milk SAA)
Storage
4°C
Species
Cow
Introduction
SAA3, is the isoform of serum amyloid A that is expressed in bovine mammary epithelial cells and secreted into milk. It is a positive acute phase protein of approximately 12 kDa. Levels increase because of inflammation and infection associated with mastitis. In studies at Veterinary Biomarkers Inc., we find that milk SAA3 levels correlate with somatic cell count (SCC).
Principle Of The Assay
The assay uses two cow SAA3 monoclonal antibodies: SAA3-11-11H3 for solid phase immobilization (microtiter wells) and HRP conjugated SAA311-5H6 for detection. Diluted milk samples and standards are incubated in microtiter wells together with HRP conjugate for one hour. If present, SAA3 molecules are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRPconjugate. TMB is added and incubated for 20 minutes. If SAA3 is present, a blue color develops. Color development is stopped by addition of Stop solution; changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA3 is proportional to absorbance and is derived
from a standard curve.
