Cat. No.
MAK-0203
Product Name
Goat SAA
Storage
2-8°C
Species
Goat
Introduction
Serum amyloid A (SAA) is a positive acute phase protein of ≈12 kDa. In goats, amino acid sequences for three isoforms have been reported at the time of writing: A3, A4 and X2. The A3 and X2 isoforms are expressed in extra hepatic tissues including mammary tissue.1 The A4 isoform is expressed in the liver.
Principle Of The Assay
One antibody is used for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), is used for detection. Standards and diluted samples are incubated, in the microtiter wells, together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived
from a standard curve.
