Cat. No.
MAK-0204
Product Name
Horse SAA
Storage
4°C
Species
Horse
Introduction
Serum amyloid A (SAA) is a positive acute phase protein of approximately 12 kDa that is expressed in lever and circulates in blood. Serum levels in horses can increase several hundredfold because of infection, disease, and tissue injury.
Principle Of The Assay
The assay uses two different peptide-specific horse SAA antibodies; one for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), for detection. Serum samples are first denatured by heating for one hour at 60°C. The denaturing step dissociates SAA from interfering factors. Subsequently, the
denatured samples are diluted. Standards and diluted samples are incubated in the microtiter wells together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The
concentration of SAA is proportional to absorbance and is derived from a standard curve.
