Cat. No.
MAK-0205
Product Name
Camel SAA
Storage
2-8°C
Species
Camel
Introduction
Serum amyloid A (SAA) is a positive acute phase protein of ≈12 kDa that is expressed in lever and circulates in blood. It is a useful biomarker of inflammation and disease in many species including camelids. This assay recognizes SAA in camel and alpaca serum. Reactivity with llama SAA has not yet been investigated.
Principle Of The Assay
The assay uses two different peptide-specific camel SAA antibodies; one for solid phase immobilization (microtiter wells) and the other, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived from a standard curve.
