Cat. No.
MAK-0206
Product Name
Dolphin SAA
Storage
4°C
Species
Dolphin
Introduction
Serum amyloid A (SAA) is a positive acute phase protein of approximately 12 kDa that is expressed in lever and circulates in blood. It serves as a useful biomarker of inflammation and general health in dolphins.
Principle Of The Assay
The assay uses two different peptide-specific dolphin SAA antibodies; one for solid phase immobilization (microtiter wells) and the other, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived from a standard curve.
