Cat. No.
MAK-0207
Product Name
Human SAA
Storage
4°C
Species
Human
Introduction
SAA is an acute phase protein of approximately 12.5 kDa that is expressed in the liver and circulates in blood. Serum levels can increase several hundred-fold during inflammation and disease.
Principle Of The Assay
The assay uses two different peptide-specific human SAA antibodies; one for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells, together with HRP conjugate for one hour. SAA molecules, if present, are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present, a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived
from a standard curve.
