Cat. No.
MAK-0208
Product Name
Elephant SAA
Storage
4°C
Species
Elephant
Introduction
Serum amyloid A (SAA) is a positive acute phase protein of approximately 12 kDa. Serum levels increase due to inflammation and infection in many species. In elephants, serum levels of 0 - 47.5 µg/ml have been reported for healthy elephants. Levels up to 410 µg/ml were found in sick elephants.
Principle Of The Assay
The assay uses two elephant SAA peptide-specific antibodies. One is used for solid phase immobilization (microtiter wells). The other, conjugated to HRP, is used for detection. Diluted serum samples and standards are incubated in microtiter wells together with HRP conjugate for one hour. If present, SAA molecules are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP conjugate. TMB is added and incubated for 20 minutes. If SAA is present, a blue color develops. Color development is stopped by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived
from a standard curve.
