Cat. No.
MAK-0209
Product Name
Mouse SAP
Storage
2-8°C
Species
Mouse
Introduction
SAP is a member of the pentraxin family of acute phase proteins that includes C-reactive protein. It circulates in blood as a decamer of a single polypeptide chain of mwt 25 kDa. It is a positive acute phase protein in all strains of mice.
Principle Of The Assay
One is used for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), is used for detection. Diluted serum samples (100 µl) are incubated in the antibody-coated microtiter wells together with HRP conjugate (100 µl) for one hour. As a result, SAP molecules are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured at 450 nm. The concentration of SAP is proportional to the optical density of the test sample and is derived from a standard curve.
