Cat. No.
MAK-0210
Product Name
Rat Thiostatin
Storage
2-8°C
Species
Rat
Introduction
Thiostatin is a cysteine protease inhibitor that is also known as major acute phase protein (α1-MAP). In response to inflammation, serum levels of thiostatin increase as much as 20-fold. Thiostatin is a useful biomarker of inflammation and disease in rats.
Principle Of The Assay
The assay uses affinity purified rat thiostatin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat thiostatin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in thiostatin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If thiostatin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of thiostatin is proportional to absorbance and is
derived from a standard curve.
