Anti-F4/80 [Cl:A3-1 (recombinant version)](MABL-4008)

MABL-4008

FC, IHC

Engineer antibody

Mouse

Cl:A3-1 (recombinant version)

Recombinant Antibody

This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein, expressed by murine macrophages.

mouse macrophage marker; EMR1 receptor; EGF-like module; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Em Gpf4; TM7; Ly-71; f480; f-480; f4 80; f 480; f- 4/80; f4-80

Q61549

The original antibody was generated by immunizing femal BALB/c mice with biotin-KLH conjugate.

The anti-F4/80 antibody, clone Cl:A3-1, has been very widely used in flow cytometry, microscopy and Western blotting since its generation by Austyn and Gordon in 1980. We are the only supplier of recombinant versions of Cl:A3-1 which, whilst having the same variable domains as the original, has been shown to block hybridoma derived anti-F4/80:APC binding to in vitro generated bone marrow- derived (BMDMs) and ex vivo splenic murine macrophages. This was tested using both the recombinant version of the original isotype (rat IgG2b) as well as a murinised version (IgG2a, Ab00106-2.0). Further testing on BMDMs shows that our Fc Silent™ rat IgG2b is a valuable new reagent which shows distinct labelling of F4/80-positive cell populations, whilst the isotype control shows no binding, suggesting that only the antigen binding domains are involved in binding, promoting precision of labelling. The murinised versions will be valuable tools in the study of the role of the murine macrophage in vivo without the artefacts rat-derived antigen being co-administered may harbour. Particularly the murine IgG2a (Ab00106- 2.0) should be an excellent candidate for macrophage depletion studies, with the Fc Silent™ (Ab00106-2.3) version being the ideal negative control. Using our recombinant production platform, any species and isotype are possible and our Support Team (support@absoluteantibody.com) will be glad to help you with finding the right variant to match your experimental needs.

Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur J Immunol. 1981 Oct;11(10):805-15. PMID:7308288

Describes the making of the antibody and its specificity for macrophages (Flow Cytometry) and proposes its potential use as a marker.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.