Cat. No.
MABL-4012
Application
IHC-F, IHC-P, IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
9E 10
From
Recombinant Antibody
Specificity
Epitope sequence, EQKLISEEDL, located in aa 410-419 of human c-myc protein.
Alternative Names
Proto-oncogene c-Myc; Transcription factor p64; Class E basic helix-loop- helix protein 39; BHLHE39; cmyc
UniProt
P01106
Immunogen
The original antibody was generated by immunizing mice with human chronic myelogenous leukemia (CML) cells.
Application Notes
Antibody First Published
Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 Dec;5(12):3610-6. PMID:3915782
Note on publication
Describes the making of the antibody, shows it recognises a 62kDa c-myc protein in c-myc overexpressing cell lines (Western blot).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
