Cat. No.
MABL-4013
Application
IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
N901
From
Recombinant Antibody
Specificity
This antibody binds CD56, present on large granular lymphocytes including natural killer cells. This antibody does not react with monocytes, granulocytes, B cells, erythrocytes, or platelets.
Alternative Names
NCAM1; NCAM; NCAM-1; Neural cell adhesion molecule 1
UniProt
P13591
Immunogen
Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.
Application Notes
This antibody does not block the natural killer cell activity and can be used to rapidly purify functional NK cells (PMID:6574190). This antibody was able to precipitate a band at approx. 200-250 kdalton protein in a immunoprecipitation assay. This antibody successfully blocked the binding of anti- NKH1A antibody in an indirect immunofluorescence assay (PMID:3884668). This antibody is also recommended to be used in the study of cell surface antigens of the natural cells (PMID: 3875369).Conjugate of DC1 with murine and humanizsed versions of N901 was found to be potent and effective in selective killing of target cells in culture (PMID: 7545085). THis antibody can also be used for the separation of natural killer cells in a flow cytometry (PMID: 15985539).
Antibody First Published
Griffin et al. Characterization of an antigen expressed by human natural killer cells. J Immunol (1983);130(6):2947-51. PMID:6574190
Note on publication
Describes the characterization of the antigen present on human natural killer cells using this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
