Cat. No.
MABL-4014
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
M2.1
From
Recombinant Antibody
Specificity
This antibody recognises the DYKDDDDK-tag, a widely used epitope tag.
Alternative Names
FLAG-tag; Equivalent to FLAG antibodies from Sigma; DYKDDDDK; DDDK; AspTyrLysAspAspAspAspLy
UniProt
Immunogen
This scFv antibody was generated by previously crystallized hexa-histidine-specific 3D5 scFv antibody. The 3D5 scFv was modified in the complementary determining region by random mutagenesis, in conjunction with phage display, to yield scFvs with new biochemical characteristics and binding specificity.
Application Notes
This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N- terminally, C-terminally as well as internally. This antibody's binding is not sensitive to calcium. This antibody recognises both native and denatured protein.
Antibody First Published
Brizzard et al. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 1994 Apr;16(4):730-5. PMID:8024796
Note on publication
Describes the characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
