Cat. No.
MABL-4015
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
EE tag
Clone No.
3D5/EE_48.K
From
Recombinant Antibody
Specificity
This antibody recognizes and binds a six amino acid long sequence (EYMPME).
Alternative Names
EE epitope tag (EYMPME); Glu-Glu epitope tag
UniProt
Immunogen
Biotinylated GCN4-peptide (HLENEVARLKK).
Application Notes
This antibody successfully identifies protein with either internal or C-terminal EE peptides in western blot assays. When expressed in soluble form they recognize the EE peptides in ELISA (PMID:21217145; PMID:24615866).
Antibody First Published
Pai et al. Conversion of scFv peptide-binding specificity for crystal chaperone development. Protein Eng Des Sel. (2011); 24(5): 419–428. PMID:21217145
Note on publication
Describes the generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
