Cat. No.
MABL-4016
Application
IP, SPR, WB
Isotype
Engineer antibody
Species Reactivity
S. cerevisiae
Clone No.
C11L34
From
Recombinant Antibody
Specificity
This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity.
Alternative Names
General Control Protein 4
UniProt
P03069
Immunogen
Peptide "YISLIYTNYEAGK" derived from Glutathione S-transferase P conjugated to KLH.
Application Notes
This antibody binds to the GCN4-leucine zipper peptide, which is part of the dimerisation- and DNA-binding domain of the transcriptional activator GCN4 in S. cerevisiae.
Antibody First Published
Zahnd et al. Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. Journal of Biological Chemistry 2004; 279(18):18870-18877 PMID:14754898
Note on publication
Describes the generation of an scFv with high affinity using directed evolution.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
