Cat. No.
MABL-4019
Application
FACS, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Species independant
Clone No.
3D5
From
Recombinant Antibody
Specificity
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Alternative Names
polyhistidine tag
UniProt
Immunogen
The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5.
Application Notes
This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A- transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).
Antibody First Published
Lindner P, Bauer K, Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R, Plückthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. PMID:8994661
Note on publication
Describes the making of the antibody, shows it recognised carboxy-terminal oligo- histidine tags (His tags) of a wide variety of proteins in immuno-assays of cell lines with His tag amplifications.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.