Cat. No.
MABL-4020
Application
immunocytochemistry, Block, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JES1-39D10
From
Recombinant Antibody
Specificity
This antibody recognizes and binds human interleukin-5.
Alternative Names
IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
UniProt
P05113
Immunogen
The original antibody was generated as a diabody by fusing two different Fv domains by short peptide linkers.
Application Notes
This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).
Antibody First Published
Denburg et al. Interleukin-5 is a human basophilopoietin: induction of histamine content and basophilic differentiation of HL-60 cells and of peripheral blood basophil-eosinophil progenitors. Blood (1991); 77(7):1462-8. PMID:1706953
Note on publication
Describes the use of this antibody to study the effect of recombinant human interleukin-5 on myeloid colony formation in methylcellulose and on alkaline passaged HL-60 basophilic cell differentiation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
