Cat. No.
MABL-4021
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
MBP-7128
From
Recombinant Antibody
Specificity
This antibody recognizes and binds maltose binding protein of E. coli.
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEX9
Immunogen
This conformation specific synthetic antibody was generated by utilizing a phage display library wherein the the Fab fragments is fused to the minor coat protein pIII of the M13 bacteriophage. To generate sABs that preferentially bind to the closed form of MBP (closed-specific sABs), the selection was carried out in the presence of 10 μM maltose. After fours rounds of selection the isolated colonies were subjected to competitive phage ELISA to provide an estimate of affinity towards MBP and specificity for closed form.
Application Notes
Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. Maltose- binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolation applications, MBP provides a high degree of translation and facilitates the proper folding and solubility of the target protein (PMID: 20978971).
Antibody First Published
Kim et al. Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Sci Rep. (2016); 6; 34515. PMID:27682821
Note on publication
Describes the crystal structure of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
