Cat. No.
MABL-4030
Application
IP, Radioimmune labeling, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Cow, Human, Zebrafish, Amphibians, Rat, Mouse
Clone No.
Rho 1D4
From
Recombinant Antibody
Specificity
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Alternative Names
Rho-tag; Visual Purple
UniProt
P08100
Immunogen
Rim ABC protein.
Application Notes
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein- coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.
Antibody First Published
Molday et al. 1983 Monoclonal antibodies to rhodopsin: characterization, corss-reactivity, and applicatoin as sturctural probes. Biochemistry, 1983; 22(3):653-660 PMID:6188482
Note on publication
Describes the generation of two monoclonal antibodies from hybridoma.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
