Cat. No.
MABL-4029
Application
epitope tag, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
PMab-2
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 38-51 (GDDMVNPGLEDRIEC) of the platelet- aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN). PDPN is a is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets
Alternative Names
Podoplanin; E11 antigen epitope; Glycoprotein 38; Gp38; OTS-8; Ots8; RTI140; T1-alpha; T1A; Type I cell 40 kDa protein
UniProt
Q64294
Immunogen
Bleached bovine rod outer segment (ROS).
Application Notes
The specificity of this antibody for endogenous rat podoplanin (PDPN) has been confirmed in ELISA, Western blot and flow cytometry analysis (Oki et al, 2015), where PMab-2 was shown not to react with human and mouse podoplanin. Flow cytometry has also been used to demonstrate the high binding affinity of this antibody (KD of PMab-2 = 5.9 × 10−10 M) (Oki et al, 2015). In immunohistochemical analysis, this antibody is highly sensitive to rat PDPN, detecting type I lung alveolar cells, colon lymphatic endothelial cells, podocytes and Bowman's capsule epithelial cells at low concentration (Oki et al, 2015).This antibody possess high affinity and specificity for the RAP epitope tag (DMVNPGLEDRIE), and can, therefore, be used to detect proteins in Western blots, flow cytometry and sandwich enzyme-linked immunosorbent assays (ELISA), as well as for protein purification. (Fujii et al, 2017).
Antibody First Published
Oki et al Development of Sensitive Monoclonal Antibody PMab-2 Against Rat Podoplanin Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. December 2015, 34(6): 396-403. PMID:26683179
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
