Cat. No.
MABL-4028
Application
Blocking, Purification, ELISA
Isotype
Engineer antibody
Species Reactivity
Baboons, Human
Clone No.
HPC-4
From
Recombinant Antibody
Specificity
Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.
Alternative Names
Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC
UniProt
P04070
Immunogen
This antibody was raised by immunising mice with 14-residue synthetic peptide rpp3851, which corresponds to amino acids 38-51 (GDDMVNPGLEDRIEC) of the platelet-aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN). Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
Application Notes
HPC-4 binds human protein C only in the presence of Ca2+; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca2+-dependent manner, purification, and others (Tanaka et al., 2006; PMID
Antibody First Published
Taylor et al. Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon. J Clin Invest. 1987 Mar;79(3):918-25. PMID:3102560
Note on publication
Describes the generation and characterisation of the HPC-4 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
