Cat. No.
MABL-4027
Application
epitope tag, FACS, WB, Block, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
PMab-1
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation- stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin. PDPN is a type I transmembrane protein that interacts with the platelet receptor C-type lectin- like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets. Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT.
Alternative Names
PDPN; Aggrus; Glycoprotein 38; Gp38; OTS-8; Ots8; PA2.26 antigen; T1- alpha; T1A; Transmembrane glycoprotein E11
UniProt
Q62011
Immunogen
This antibody was raised by immunising mice with human protein C.
Application Notes
This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012).
Antibody First Published
Kaji et al Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin Acta Histochem. Cytochem. 45 (4): 227–237, 2012. doi:10.1267/ahc.12008 PMID:23012488
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
