Cat. No.
MABL-4026
Application
inhibit, Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2E 6
From
Recombinant Antibody
Specificity
This antibody binds human notch 1.
Alternative Names
Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
UniProt
P46531
Immunogen
This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
Application Notes
The original human IgG1 version of this antagonist antibody binds notch 1 with an affinity of Kd= 8 nM. This antibody is capable of efficiently blocking the binding of all 4 ligands (Jag1, Jag2, DLL1 and DLL4) to notch 1. This antibody was also capable of specifically inhibiting Notch 1-dependent transcription. In vivo, this antibody was found to show little to no intestinal toxicity in mice. Hu2E6-62 caused decrease in functional angiogenesis by increasing vascular branching and decreasing vascular function. It inhibited angiogenesis induced by the human lung cancer Calu-6 cells. This antibody can be used to inhibit or reduced the proliferation and growth of tumors (WO2012003472).
Antibody First Published
WO2012003472
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
