Cat. No.
MABL-4025
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
sAb-P1
From
Recombinant Antibody
Specificity
This antibody is a conformation specific antibody and binds the closed conformation of MBP across the face of the closed binding pocket, effectively trapping the maltose inside. It does not bind the open conformation of the MBP. This antibody binds the peristeric site of MBP with its epitope spanning across the two lobes that form the entrance to the maltose-binding pocket. A short α-helix comprising residues 64–73 on one lobe of MBP and the long helix formed by residues 334–352 on its other lobe, form part of the extended interface between sAB-P1 and MBP. These two helices have a remarkably different relative orientation in the open and closed forms of MBP, thereby imparting conformational specificity of sAB-P1.
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEY0; P0AEX9
Immunogen
The original antibody was generated by immunizing BALB/c mice with a concatemeric protein containing 4 repeats of amino acids 413-488 of human Notch 1 linked by two non-Notch 1 amino acids (AG linker).
Application Notes
The affinity of maltose towards MBP is enhanced more than 1000-fold in the presence of an excess of sAB-P1. However, it is reported to inhibit the maltose transport by the Escherichia coli maltose transporter, MalFGK2, across the periplasm. This is because the epitope for sAB-P1 is different from the other sABs (sAB-7O and sAB-11M). It is also confirmed that sAB-P1 provides additional stabilization of the maltose-bound state (PMID: 29321208).
Antibody First Published
Mukherjee et al. Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem. (2018); 293(8); 2815-2828. PMID:29321208
Note on publication
Describes the conformational structure and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
