Cat. No.
MABL-4024
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
sAb-70
From
Recombinant Antibody
Specificity
This antibody is a conformation specific antibody and binds the maltose binding protein in its open conformation. It is reported that sAB-7O binds a large endosteric site on MBP using its heavy-chain CDR-H1, -H2, and -H3 and light chain CDR-L1 and -L3 residues.
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEY0; P0AEX9
Immunogen
This conformationally selective synthetic antibody was generated by customized phage display selections that stabilize MBP in different conformational states.
Application Notes
This antibody binds MBP with KD of 1 nM in the absence of maltose and 152 nM in 1 µM maltose and does not bind in 1 mM maltose. This antibody binds to the maltose binding pocket of MBP with sufficient affinity to effectively outcompete maltose binding and driving the equilibrium to the open form. Therefore, in presence of excess sAB-7O there was no appreciable observation of maltose binding. Competitive ELISA was used to determine that the open (sAB-7O) and closed conformation (sAB-11M) specific antibodies compete for binding site of MBP (PMID: 29321208).
Antibody First Published
Mukherjee et al. Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem. (2018); 293(8); 2815-2828. PMID:29321208
Note on publication
Describes the conformational structure and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
