Cat. No.
MABL-4023
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Escherichia coli
Clone No.
sAb-11M
From
Recombinant Antibody
Specificity
This antibody is a conformation specific antibody and binds the maltose binding protein in its closed conformation. This antibody is reported to binf the hinge region of the MBP which lies in the opposite direction of the maltose-binding pocket..
Alternative Names
malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
UniProt
P0AEY0; P0AEX9
Immunogen
This conformationally selective synthetic antibody was generated by customized phage display selections that stabilize MBP in different conformational states.
Application Notes
This antibody binds MBP with affinity of 20nM in presence of 1 µM maltose. Upon binding to MBP, sAB-11M shifts the MBP conformation to the closed form, thereby covering/altering the epitope and preventing the binding of antibodies specific for open confirmation of the MBP (e.g. sAB-7O). It is reported that in the presence of a 5-fold excess of sAB-11M increases the affinity for maltose by 120-fold. Competitive ELISA was used to determine that the open (sAB-7O) and closed conformation (sAB-11M) specific antibodies compete for binding site of MBP (PMID: 29321208).
Antibody First Published
Mukherjee et al. Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem. (2018); 293(8); 2815-2828. PMID:29321208
Note on publication
Describes the conformational structure and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
