Cat. No.
MABL-4033
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Streptomyces avidinii
Clone No.
C23.21
From
Recombinant Antibody
Specificity
This antibody recognizes an antigen with amino acid sequence 'WSHPQFEK' present in the Strep Tag with a dissociation constant of 1.4x10^-10M. It recognizes another fusion protein namely VHH-E9 containing the strep-tag sequence of 'LESAWSHPQFEK' at the C terminus with a dissociation constant of 9.1x10^-10M.
Alternative Names
Streptavidin; 'WSHPQFEK'; strep-tag
UniProt
P22629
Immunogen
Mice were injected with bacterially expressed full-length Xenopus laevis Orc1p, and spleen cells from three best-responding mice were used to generate antibody-producing hybridomas. Antibodies were screened by enzyme-linked immunosorbent assay and immunoprecipitation.
Application Notes
This high affinity antibody can detect the strep-tag II fusion proteins in ELISA and western blot assay. This antibody is recommended for strep-tag recognition in fusion proteins through western blotting or immunohistochemistry methods, or to immobilize strep-tagged fusion proteins on Biacore sensor chips for performing Surface Plasmon Resonance (SPR)-mediated protein interaction studies. It can also be used for the purification of strep-tagged recombinant proteins using an affinity column or immunoprecipitation meothod.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
