Cat. No.
MABL-4034
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Xenopus laevis
Clone No.
TK 15
From
Recombinant Antibody
Specificity
Recognises recombinant proteins TK15 tag at the carboxy terminus. Amino terminal and internal TK15 tags have not been tested.
Alternative Names
TK-15 tag
UniProt
Immunogen
Pk (V5) Epitope Tag (GKPIPNPLLGLDST).
Application Notes
This antibody was first used to isolate Xenopus origin recognition complex peptides (Tugal 1998) but can now be used for the purification and detection of recombinant fusion proteins incorporating a single eight amino acid TK15 tag.
Antibody First Published
Tugal et al. The Orc4p and Orc5p Subunits of the Xenopus and Human Origin Recognition Complex Are Related to Orc1p and Cdc6p Biol Chem. 273(49):32421-9 (1998) PMID:9829972
Note on publication
Outlines the purification of Xenopus ORC for characterization of ORC and the study of its interaction with DNA to look for DNA sequence specificity in origin function.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
