Name
HRP
Cat. No.
MAG-3427
Tag/Conjugates
His
Source
Root extracts of horseradish.
Shipped
At Room Temperature
Description
HRP consists of the basic isoenzyme having a molecular weight of 44 kDa. The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Introduction
The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blottingand ELISAs. HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
Purification Method
/
Physical Appearance
Sterile Filtered red-brown lyophilized powder.
Formulation
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Stability
Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
(A403/A275) = RZ: 3.0.
Biological Activity
276 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H 2 O 2 as substrates).
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Horseradish Peroxidase, HRP, EC 1.11.1.7.
Amino acid sequence
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Background
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