Cat. No.
MAK-0086
Product Name
Mouse IgM
Storage
4°C
Species
Mouse
Instructions
The mouse IgM ELISA kit is designed for measurement of IgM in mouse serum or plasma.
Introduction
The mouse IgM ELISA kit is designed for measurement of IgM in mouse serum or plasma. The assay uses goat anti-mouse IgM for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-mouse IgM antibodies for detection. Both capture and detection antibodies were cross absorbed on mouse IgG and IgA agarose columns, thereby ensuring specificity for IgM. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared mouse IgM standards. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. IgM molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgM is proportional to the optical density of the test sample and is derived from a standard curve.
